DNA scaffold produces diamond-like nanomaterial

Using bundled strands of DNA to build tetrahedral cages, scientists at the US Department of Energy's Brookhaven National Laboratory have devised a way to trap and arrange nanoparticles in a way that mimics the crystalline structure of diamond. This research, detailed in a paper in Science, may open a path to new materials that take advantage of the optical and mechanical properties of this crystalline structure for applications such as optical transistors.

"We solved a 25-year challenge in building diamond lattices in a rational way via self-assembly," said Oleg Gang, a physicist at the Center for Functional Nanomaterials (CFN) at Brookhaven Lab. Gang led this research in collaboration with scientists from Stony Brook University, Wesleyan University and Nagoya University in Japan.

The scientists employed a technique developed by Gang that uses fabricated DNA as a building material to organize nanoparticles into 3D spatial arrangements. They used rope-like bundles of double-helix DNA to create rigid, three-dimensional frames, and then added dangling bits of single-stranded DNA that can bind nanoparticles coated with complementary DNA strands.

"We're using precisely shaped DNA constructs made as a scaffold and single-stranded DNA tethers as a programmable glue that matches up particles according to the pairing mechanism of the genetic code – A binds with T, G binds with C," outlined Wenyan Liu of the CFN, the lead author on the paper. "These molecular constructs are building blocks for creating crystalline lattices made of nanoparticles."

"Building diamond superlattices from nano- and micro-scale particles by means of self-assembly has proven remarkably difficult, " Liu added. "It challenges our ability to manipulate matter on small scales."

This difficulty is mainly a result of structural features such as a low packing fraction – in contrast to many other crystalline structures, particles in a diamond lattice occupy only a small part of the lattice volume – and strong sensitivity to the way bonds between particles are oriented. "Everything must fit together in just such a way without any shift or rotation of the particles' positions," Gang said. "Since the diamond structure is very open, many things can go wrong, leading to disorder."

"Even to build such structures one-by-one would be challenging," Liu asserted, "and we needed to do so by self-assembly because there is no way to manipulate billions of nanoparticles one-by-one."

Gang's previous success using DNA to construct a wide range of nanoparticle arrays suggested that a DNA-based approach might work in this instance. The team first used the rope-like DNA bundles to build tetrahedral ‘cages’. They then added single-stranded DNA tethers pointing toward the interior of the cages, using T,G,C,A sequences that matched up with complementary tethers attached to gold nanoparticles. When mixed in solution, the complementary tethers paired up to ‘trap’ one gold nanoparticle inside each tetrahedron cage.

The arrangement of gold nanoparticles outside the cages was guided by a different set of DNA tethers, attached at the vertices of the tetrahedrons, which bound with complementary DNA tethers attached to a second set of gold nanoparticles. When mixed and annealed, the tetrahedral arrays formed superlattices with long-range order, in which the positions of the gold nanoparticles mimic the arrangement of carbon atoms in a lattice of diamond, but at a scale about 100 times larger.

"Although this assembly scenario might seem hopelessly unconstrained, we demonstrate experimentally that our approach leads to the desired diamond lattice, drastically streamlining the assembly of such a complex structure," Gang said.

The proof is in the images. The scientists used cryogenic transmission electron microscopy (cryo-TEM) to verify the formation of tetrahedral frames by reconstructing their 3D shape from multiple images. Then they used in-situ small-angle x-ray scattering (SAXS) at the National Synchrotron Light Source and cryo scanning transmission electron microscopy (cryo-STEM) at the CFN to image the arrays of nanoparticles in the fully-constructed lattice.

"Our approach relies on the self-organization of the triangularly-shaped blunt vertices of the tetrahedra (so called 'footprints') on isotropic spherical particles, " explained Gang. "Those triangular footprints bind to spherical particles coated with complementary DNA, which allows the particles to coordinate their arrangement in space relative to one another. However, the footprints can arrange themselves in a variety of patterns on a sphere. It turns that one particular placement is more favorable, and it corresponds to the unique 3D placement of particles that locks the diamond lattice."

The team supported their interpretation of the experimental results using theoretical modeling that provided insights into the main factors driving the successful formation of diamond lattices.

"This work brings to the nanoscale the crystallographic complexity seen in atomic systems," said Gang, who noted that the method can readily be expanded to organize particles of different material compositions. The group has previously demonstrated that DNA-assembly methods can be applied to optical, magnetic and catalytic nanoparticles as well, and will likely yield novel optical and mechanical materials.

"We've demonstrated a new paradigm for creating complex 3D-ordered structures via self-assembly. If you can build this challenging lattice, the thinking is you can build potentially a variety of desired lattices, " he concluded.

This story is adapted from material from Brookhaven National Laboratory, with editorial changes made by Materials Today. The views expressed in this article do not necessarily represent those of Elsevier. Link to original source.